An "in house" system for protein crystallography
The G-Rob system delivered to the EPFL is a fully automated system that includes:
- a Rigaku MicroMax-007HF rotating anode
- a Photonic Science 165mm CCD detector
- a Oxford Cryosystem 700 Series Cryostream Plus
- the G-Rob 1D "goniometer" function
- the G-Rob 2D "plate screening" function
- the G-Rob 1DT "frozen samples changer" function
- the G-Rob "beam monitor" function
Tests accomplished with frozen samples
Before delivery, numerous dataset were collected on frozen samples
by crystallographers invited to evaluate the system.
E. coli ADC lyase
180 deg (0.5 deg per frame) dataset were collected on a E. coli ADC lyase crystal (left image, below).
Good diffraction was observed up to 2.3 A, with only 2 mn exposure time (right image, below).
Integration revealed good statistics, with 16.7% Rsym and 8.6 I/sigma
in the last resolution shell (2.45-2.3 A). The resulting 2fo-fc is displayed below
(courtesy of D. Cobessi, IBS).